2024 crispr cas9 sgrna library design Search Results


crispr cas9  (Kimble Inc)
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2024 crispr cas9 sgrna library design  (Addgene inc)
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crispr/cas9-mediated genome editing  (SunyBiotech Corporation)
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cas9  (Addgene inc)
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Addgene inc cas9
Figure 5. Regulation of p53 signaling by the MDM2 30 UTR Alu cluster (A and B) Schematic of <t>Cas9-mediated</t> genomic deletion of IRR (DIRR; A) or neighboring deletion of MDM2 (DIRR-NR; B). PCR amplicons using genomic DNA (gDNA) extracted from A549 WT, DIRR, or DIRR-NR analyzed on a 1% TAE agarose gel are shown together. The expected PCR amplicons are shown on the right. (C) RNA-FISH analysis of MDM2 mRNA in A549 WT, DIRR, or DIRR-NR transfected with siCSTF2&T and its colocalization rate with DAPI (n = 5). (D and E) Repression of MDM2 expression and activation of the p53 pathway analyzed by western blotting (D) and RT-qPCR (E; n = 3) in A549 WT, DIRR, or DIRR-NR.
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crispr/cas9 screens  (Broad Institute Inc)
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Broad Institute Inc crispr/cas9 screens
Figure 5. Regulation of p53 signaling by the MDM2 30 UTR Alu cluster (A and B) Schematic of <t>Cas9-mediated</t> genomic deletion of IRR (DIRR; A) or neighboring deletion of MDM2 (DIRR-NR; B). PCR amplicons using genomic DNA (gDNA) extracted from A549 WT, DIRR, or DIRR-NR analyzed on a 1% TAE agarose gel are shown together. The expected PCR amplicons are shown on the right. (C) RNA-FISH analysis of MDM2 mRNA in A549 WT, DIRR, or DIRR-NR transfected with siCSTF2&T and its colocalization rate with DAPI (n = 5). (D and E) Repression of MDM2 expression and activation of the p53 pathway analyzed by western blotting (D) and RT-qPCR (E; n = 3) in A549 WT, DIRR, or DIRR-NR.
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crispr cas9 components  (Exosome Diagnostics)
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Exosome Diagnostics crispr cas9 components
Figure 5. Regulation of p53 signaling by the MDM2 30 UTR Alu cluster (A and B) Schematic of <t>Cas9-mediated</t> genomic deletion of IRR (DIRR; A) or neighboring deletion of MDM2 (DIRR-NR; B). PCR amplicons using genomic DNA (gDNA) extracted from A549 WT, DIRR, or DIRR-NR analyzed on a 1% TAE agarose gel are shown together. The expected PCR amplicons are shown on the right. (C) RNA-FISH analysis of MDM2 mRNA in A549 WT, DIRR, or DIRR-NR transfected with siCSTF2&T and its colocalization rate with DAPI (n = 5). (D and E) Repression of MDM2 expression and activation of the p53 pathway analyzed by western blotting (D) and RT-qPCR (E; n = 3) in A549 WT, DIRR, or DIRR-NR.
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cas9 mrna  (TriLink)
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TriLink cas9 mrna
Figure 5. Regulation of p53 signaling by the MDM2 30 UTR Alu cluster (A and B) Schematic of <t>Cas9-mediated</t> genomic deletion of IRR (DIRR; A) or neighboring deletion of MDM2 (DIRR-NR; B). PCR amplicons using genomic DNA (gDNA) extracted from A549 WT, DIRR, or DIRR-NR analyzed on a 1% TAE agarose gel are shown together. The expected PCR amplicons are shown on the right. (C) RNA-FISH analysis of MDM2 mRNA in A549 WT, DIRR, or DIRR-NR transfected with siCSTF2&T and its colocalization rate with DAPI (n = 5). (D and E) Repression of MDM2 expression and activation of the p53 pathway analyzed by western blotting (D) and RT-qPCR (E; n = 3) in A549 WT, DIRR, or DIRR-NR.
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dead cas9  (Addgene inc)
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Addgene inc dead cas9
Figure 5. Regulation of p53 signaling by the MDM2 30 UTR Alu cluster (A and B) Schematic of <t>Cas9-mediated</t> genomic deletion of IRR (DIRR; A) or neighboring deletion of MDM2 (DIRR-NR; B). PCR amplicons using genomic DNA (gDNA) extracted from A549 WT, DIRR, or DIRR-NR analyzed on a 1% TAE agarose gel are shown together. The expected PCR amplicons are shown on the right. (C) RNA-FISH analysis of MDM2 mRNA in A549 WT, DIRR, or DIRR-NR transfected with siCSTF2&T and its colocalization rate with DAPI (n = 5). (D and E) Repression of MDM2 expression and activation of the p53 pathway analyzed by western blotting (D) and RT-qPCR (E; n = 3) in A549 WT, DIRR, or DIRR-NR.
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Image Search Results


Figure 5. Regulation of p53 signaling by the MDM2 30 UTR Alu cluster (A and B) Schematic of Cas9-mediated genomic deletion of IRR (DIRR; A) or neighboring deletion of MDM2 (DIRR-NR; B). PCR amplicons using genomic DNA (gDNA) extracted from A549 WT, DIRR, or DIRR-NR analyzed on a 1% TAE agarose gel are shown together. The expected PCR amplicons are shown on the right. (C) RNA-FISH analysis of MDM2 mRNA in A549 WT, DIRR, or DIRR-NR transfected with siCSTF2&T and its colocalization rate with DAPI (n = 5). (D and E) Repression of MDM2 expression and activation of the p53 pathway analyzed by western blotting (D) and RT-qPCR (E; n = 3) in A549 WT, DIRR, or DIRR-NR.

Journal: Molecular cell

Article Title: Alternative polyadenylation determines the functional landscape of inverted Alu repeats.

doi: 10.1016/j.molcel.2024.01.008

Figure Lengend Snippet: Figure 5. Regulation of p53 signaling by the MDM2 30 UTR Alu cluster (A and B) Schematic of Cas9-mediated genomic deletion of IRR (DIRR; A) or neighboring deletion of MDM2 (DIRR-NR; B). PCR amplicons using genomic DNA (gDNA) extracted from A549 WT, DIRR, or DIRR-NR analyzed on a 1% TAE agarose gel are shown together. The expected PCR amplicons are shown on the right. (C) RNA-FISH analysis of MDM2 mRNA in A549 WT, DIRR, or DIRR-NR transfected with siCSTF2&T and its colocalization rate with DAPI (n = 5). (D and E) Repression of MDM2 expression and activation of the p53 pathway analyzed by western blotting (D) and RT-qPCR (E; n = 3) in A549 WT, DIRR, or DIRR-NR.

Article Snippet: Molecular Cell 84, 1062–1077.e1–e9, March 21, 2024 e6 Gene deletion using CRISPR-Cas9 To generate A549 cells stably expressing Cas9, a lentivirus was generated using lentiCas9-Blast (Addgene) and transduced into A549 cells.

Techniques: Agarose Gel Electrophoresis, Transfection, Expressing, Activation Assay, Western Blot, Quantitative RT-PCR